47614

Sigma-Aldrich

Fluram

BioReagent, suitable for fluorescence, ≥99.0% (UV)

Synonym(s):
Fluorescamine, 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione
Empirical Formula (Hill Notation):
C17H10O4
CAS Number:
Molecular Weight:
278.26
Beilstein/REAXYS Number:
921143
EC Number:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.

Quality Level

product line

BioReagent

assay

≥99.0% (UV)

mp

153-157 °C (lit.)
153-157 °C

solubility

acetonitrile: soluble
ethanol: soluble

fluorescence

λex 234 nm
λex 390 nm; λem 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)

suitability

suitable for fluorescence

SMILES string

O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14

InChI

1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H

InChI key

ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
José Pedro Castro et al.
Redox biology, 21, 101108-101108 (2019-01-21)
Aging is accompanied by the accumulation of oxidized proteins. To remove them, cells employ the proteasomal and autophagy-lysosomal systems; however, if the clearance rate is inferior to its formation, protein aggregates form as a hallmark of proteostasis loss. In cells...
Yusuke Suzuki et al.
Journal of agricultural and food chemistry, 56(22), 10811-10816 (2008-11-06)
Photoinduced decarboxylation via homolytic cleavage of the ester linkage generating two benzyl radicals being recoupled is known to be a major photolytic pathway of the insecticide fenvalerate in aqueous or organic solvents. A highly sensitive and selective fluorescence spectroscopic method...
Andrew D Aubrey et al.
Astrobiology, 8(3), 583-595 (2008-08-06)
The Urey organic and oxidant detector consists of a suite of instruments designed to search for several classes of organic molecules in the martian regolith and ascertain whether these compounds were produced by biotic or abiotic processes using chirality measurements....
Claire E Stanley et al.
Chimia, 66(3), 88-98 (2012-05-02)
This account highlights some of our recent activities focused on developing microfluidic technologies for application in high-throughput and high-information content chemical and biological analysis. Specifically, we discuss the use of continuous and segmented flow microfluidics for artificial membrane formation, the...
Khin Yin Win et al.
The Analyst, 137(10), 2328-2332 (2012-03-13)
Here we report a fast and effective method to visualize interactive proteins across intact mammalian cells via on-site formation of fluorescence using instant reaction of non-fluorescent fluorescamine with primary amines on proteins. Without interference by fluorescence background, this fluorogenic labelling...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.