BioReagent, suitable for fluorescence, ≥99.0% (UV)

Fluorescamine, 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.

Quality Level

product line



≥99.0% (UV)


153-157 °C (lit.)
153-157 °C


acetonitrile: soluble
ethanol: soluble


λex 234 nm
λex 390 nm; λem 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)


suitable for fluorescence

SMILES string




InChI key


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Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.


Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
José Pedro Castro et al.
Redox biology, 21, 101108-101108 (2019-01-21)
Aging is accompanied by the accumulation of oxidized proteins. To remove them, cells employ the proteasomal and autophagy-lysosomal systems; however, if the clearance rate is inferior to its formation, protein aggregates form as a hallmark of proteostasis loss. In cells...
Yusuke Suzuki et al.
Journal of agricultural and food chemistry, 56(22), 10811-10816 (2008-11-06)
Photoinduced decarboxylation via homolytic cleavage of the ester linkage generating two benzyl radicals being recoupled is known to be a major photolytic pathway of the insecticide fenvalerate in aqueous or organic solvents. A highly sensitive and selective fluorescence spectroscopic method...
Andrew D Aubrey et al.
Astrobiology, 8(3), 583-595 (2008-08-06)
The Urey organic and oxidant detector consists of a suite of instruments designed to search for several classes of organic molecules in the martian regolith and ascertain whether these compounds were produced by biotic or abiotic processes using chirality measurements....
Claire E Stanley et al.
Chimia, 66(3), 88-98 (2012-05-02)
This account highlights some of our recent activities focused on developing microfluidic technologies for application in high-throughput and high-information content chemical and biological analysis. Specifically, we discuss the use of continuous and segmented flow microfluidics for artificial membrane formation, the...
Khin Yin Win et al.
The Analyst, 137(10), 2328-2332 (2012-03-13)
Here we report a fast and effective method to visualize interactive proteins across intact mammalian cells via on-site formation of fluorescence using instant reaction of non-fluorescent fluorescamine with primary amines on proteins. Without interference by fluorescence background, this fluorogenic labelling...

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