59746

Sigma-Aldrich

Atto Rho14

NACRES:
NA.32
Pricing and availability is not currently available.

fluorescence

λex 625 nm; λem 646 nm

Quality Level

storage temp.

−20°C

General description

Atto Rho14 is a new bright rhodamine featuring a functionality for coupling to biomolecules such as DNA, RNA or proteins. The label shows strong absorption, and extraordinarily high fluorescence quantum yield. In addition the dye exhibits an exceptionally high photostability. After coupling to a substrate Atto Rho14 carries a net electrical charge of +1.
Absorption and fluorescence are pH-independent in the range of pH 2 to 11, used in typical applications. The dye is moderately hydrophilic.

Application

Atto Rho14 is a new bright rhodamine featuring a functionality for coupling to biomolecules such as DNA, RNA or proteins. The label shows strong absorption, and extraordinarily high fluorescence quantum yield. In addition the dye exhibits an exceptionally high photostability. After coupling to a substrate Atto Rho14 carries a net electrical charge of +1.
Absorption and fluorescence are pH-independent in the range of pH 2 to 11, used in typical applications. The dye is moderately hydrophilic.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Pingwu Du et al.
Inorganic chemistry, 49(23), 10753-10755 (2010-10-30)
We describe ZRL1, a turn-on colorimetric and red fluorescent zinc ion sensor. The Zn(2+)-promoted ring opening of the rhodamine spirolactam ring in ZRL1 evokes a 220-fold fluorescence turn-on response. In aqueous media, ZRL1 turn-on luminescence is highly selective for Zn(2+)...
Ha Na Kim et al.
Chemical Society reviews, 37(8), 1465-1472 (2008-07-24)
This tutorial review focuses on the recent development of rhodamine derivatives, in which the spirolactam (non-fluorescent) to ring-opened amide (fluorescent) process was utilized.
Johann Grognux et al.
Molecular bioSystems, 2(10), 492-498 (2007-01-12)
A lipase substrate microarray was obtained by printing aliphatic C2-C12 monoesters of (5R)- and (5S)-3-(5,6-dihydroxyhexyloxy)benzaldehyde by reductive alkylation on amine-functionalized glass slides coated with bovine serum albumin and a short PEG linker. The microarray features 12 substrates and their 66...
Yu Kushida et al.
Bioorganic & medicinal chemistry letters, 22(12), 3908-3911 (2012-05-23)
We have developed a novel red fluorescent dye, 2Me SiR600 (λ(em)=613 nm), in which the O atom of Rhodamine Green at the 10 position of the xanthene moiety is replaced with a Si atom, as a scaffold for probes to...

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