70706

Sigma-Aldrich

Atto 520

BioReagent, suitable for fluorescence, ≥90.0% (HPCE/HPLC)

Empirical Formula (Hill Notation):
C22H27ClN2O7
Molecular Weight:
466.91
MDL number:
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.

product line

BioReagent

assay

≥90.0% (HPCE/HPLC)

fluorescence

λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

O-Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=NH+\CC)\C(C)=CC3=C(CCC(O)=O)c2cc1C

InChI

1S/C22H26N2O3.ClHO4/c1-5-23-18-11-20-16(9-13(18)3)15(7-8-22(25)26)17-10-14(4)19(24-6-2)12-21(17)27-20;2-1(3,4)5/h9-12,23H,5-8H2,1-4H3,(H,25,26);(H,2,3,4,5)/b24-19-;

InChI key

YWLGQYDVSLBBTP-BMGIYVBOSA-N

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Application

Atto fluorescent labels are designed for high sensitivity applications, including single-molecule detection. Atto labels have rigid structures that do not show any cis-trans isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 520 shows a molar extinction of 110,000 and QY of 90% in water (95% in ethanol). Decay time is 3.6 ns.

Other Notes

Employed in confocal fluorescence microscopy to confirm formation of polymer nanoshells; using combined imaging and scanning fluorescence correlation spectroscopy to characterize microsecond emission fluctuation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Alexander B Artyukhin et al.
Langmuir : the ACS journal of surfaces and colloids, 20(4), 1442-1448 (2005-04-05)
Carbon nanotubes have been featured prominently in the nanotechnology research for some time, yet robust strategies for noncovalent chemical modification of the nanotube surface are still missing. Such strategies are essential for the creation of functional device architectures. Here, we...
Ying Xiao et al.
Analytical chemistry, 77(1), 36-46 (2004-12-30)
In this report, a combined imaging and fluorescence correlation spectroscopy (FCS) method is described and its ability to characterize microsecond fluctuations in the fluorescence emission of a sample is demonstrated. A sample scanning laser confocal microscope is operated in the...
Manoj Kumbhakar et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 10(4), 629-633 (2009-01-30)
Complex dynamics of combined long-range fluorescence resonance energy transfer (FRET) and short-range electron transfer (ET) processes in a single double-stranded DNA (dsDNA) molecule (see figure) reveals that FRET remains almost unaltered in the presence of ET. Present systems also demonstrate...
Vladimir Mekler et al.
The Journal of biological chemistry, 286(1), 270-279 (2010-10-19)
Promoter recognition by RNA polymerase is a key point in gene expression and a target of regulation. Bacterial RNA polymerase binds promoters in the form of the holoenzyme, with the σ specificity subunit being primarily responsible for promoter recognition. Free...
Christopher A Teske et al.
The journal of physical chemistry. B, 109(28), 13811-13817 (2006-07-21)
Confocal laser scanning microscopy (CLSM) is being increasingly used for observing protein uptake in porous chromatography resins. Recent CLSM studies have revealed the possible existence of a nondiffusive protein transport mechanism. Observing protein uptake with CLSM requires labeling the protein...

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