R J Clarke et al.
Biochimica et biophysica acta, 981(2), 326-336 (1989-06-06)
A method is described for studying the coupling ratio of the Na+/K+ pump, i.e., the ratio of pump-mediated fluxes of Na+ and K+, in a reconstituted system. The method is based on the comparison of the pump-generated current with the...
Biochemical and biophysical research communications, 160(2), 801-807 (1989-04-28)
In liposomes with reconstituted shark Na,K-ATPase produced to contain sucrose addition of external Na+ and ATP induce an uncoupled Na+-efflux on inside-out oriented pumps which can be inhibited by digitoxigenin. This flux mode is found to be electrogenic and accompanied...
X Yu et al.
Biophysical journal, 64(4), 1232-1242 (1993-04-01)
The Ca2+ transport adenosine triphosphatase of sarcoplasmic reticulum was reconstituted in unilamellar liposomes prepared by reverse-phase evaporation. The size of the resulting proteoliposomes was similar to that of native sarcoplasmic reticulum vesicles, but their protein content was much lower, with...
J Fumero et al.
Biochimica et biophysica acta, 944(2), 164-176 (1988-10-06)
Differential scanning calorimetry (DSC) has been employed to determine the effect of five commonly employed extrinsic potential-sensitive probes on phase transitions of multilamellar suspensions of L-alpha-dimyristoylphosphatidylcholine (DMPC). At mol% values of less than five, the effect of these probes on...
Potential-sensitive molecular probes in membranes of bioenergetic relevance.
J C Smith
Biochimica et biophysica acta, 1016(1), 1-28 (1990-03-15)