A0847

Sigma-Aldrich

Adenylyl Cyclase Toxin from Bordetella pertussis

≥70% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, ≥50 units/mg protein

MDL number:
Pricing and availability is not currently available.

Quality Level

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

177 kDa by amino acid sequence

storage temp.

2-8°C

Gene Information

human ... CAMP(820)
mouse ... CAMP(12796)
rat ... CAMP(316010)

Biochem/physiol Actions

Adenylate cyclase toxin from Bordetella pertussis belongs to the family of pore-forming toxins. It converts host ATP to cyclic AMP (cAMP). May be used to affect intracellular cAMP levels in mammalian cells.

Unit Definition

One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C

Reconstitution

When reconstituted with 50 μL of water, the protein is in 0.05 M Tris, 8 M urea, 0.002 M EDTA, pH 8.0.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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