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A1804

Sigma-Aldrich

Monoclonal Anti-Actin (Amoeba) antibody produced in mouse

clone KJ43A, ascites fluid

MDL number:

biological source

mouse

antibody form

ascites fluid

clone

KJ43A, monoclonal

contains

15 mM sodium azide

species reactivity

chicken, bovine, frog, human, Acanthamoeba castellani, pig, rabbit

application(s)

western blot: 1:100

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Specificity

This antibody recognizes the actin epitope present in erythrocytes, gizzard, skeletal, and cardiac muscle by immunoblotting. The product shows no cross-reaction with actin from Naegleria gruberi.

Immunogen

actin from Amoeba proteus cytosol.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Certificate of Analysis

Certificate of Origin

T I Ahn et al.
The Journal of protozoology, 36(6), 560-562 (1989-11-01)
The reactivity of a monoclonal antibody against actin of Amoeba proteus with actins from other sources was examined. The monoclonal antibody cross-reacted with actins from vertebrate muscles, human erythrocytes, and Acanthamoeba castellanii, but it did not react with Naegleria gruberi...
M Trebak et al.
Cellular immunology, 188(2), 151-157 (1998-10-03)
Murine acquired immunodeficiency syndrome (MAIDS) can be viewed as a lymphoproliferative disease which involves B cells as well as T cells from spleen and lymph nodes while thymus and Peyer's patches do not participate in the process. The 120-kDa protooncogene...
L Rønnov-Jessen et al.
The Journal of clinical investigation, 95(2), 859-873 (1995-02-01)
The origin of myofibroblasts in stromal reaction has been a subject of controversy. To address this question definitively, we developed techniques for purification and characterization of major stromal cell types. We defined a panel of markers that could, in combination...
Cécile Lambert et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 22(9), 1350-1361 (2007-05-16)
The mechanisms of IL-1beta stimulation of OPG were studied in more detail. Whereas p38 and ERK activation was confirmed to be needed, NF-kappaB was not necessary for this regulation. We also found that OPG production after IL-1beta stimulation was not...
C F Deroanne et al.
Cardiovascular research, 49(3), 647-658 (2001-02-13)
This investigation aimed at determining the importance of the rigidity of the adhesive support and the participation of the cytoskeleton in tubulogenesis of endothelial cells in vitro. The morphotype, biosynthetic phenotype and cytoskeleton organization of human umbilical vein endothelial cells...

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