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A1804

Sigma-Aldrich

Monoclonal Anti-Actin (Amoeba) antibody produced in mouse

clone KJ43A, ascites fluid

MDL number:

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

clone

KJ43A, monoclonal

contains

15 mM sodium azide

species reactivity

chicken, bovine, frog, human, Acanthamoeba castellani, pig, rabbit

technique(s)

western blot: 1:100

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

Specificity

This antibody recognizes the actin epitope present in erythrocytes, gizzard, skeletal, and cardiac muscle by immunoblotting. The product shows no cross-reaction with actin from Naegleria gruberi.

Immunogen

actin from Amoeba proteus cytosol.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

M Trebak et al.
Cellular immunology, 188(2), 151-157 (1998-10-03)
Murine acquired immunodeficiency syndrome (MAIDS) can be viewed as a lymphoproliferative disease which involves B cells as well as T cells from spleen and lymph nodes while thymus and Peyer's patches do not participate in the process. The 120-kDa protooncogene
L Rønnov-Jessen et al.
The Journal of clinical investigation, 95(2), 859-873 (1995-02-01)
The origin of myofibroblasts in stromal reaction has been a subject of controversy. To address this question definitively, we developed techniques for purification and characterization of major stromal cell types. We defined a panel of markers that could, in combination
Cécile Lambert et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 22(9), 1350-1361 (2007-05-16)
The mechanisms of IL-1beta stimulation of OPG were studied in more detail. Whereas p38 and ERK activation was confirmed to be needed, NF-kappaB was not necessary for this regulation. We also found that OPG production after IL-1beta stimulation was not
T I Ahn et al.
The Journal of protozoology, 36(6), 560-562 (1989-11-01)
The reactivity of a monoclonal antibody against actin of Amoeba proteus with actins from other sources was examined. The monoclonal antibody cross-reacted with actins from vertebrate muscles, human erythrocytes, and Acanthamoeba castellanii, but it did not react with Naegleria gruberi
Vincent J de Beer et al.
American journal of physiology. Heart and circulatory physiology, 301(3), H1080-H1089 (2011-06-21)
Myocardial infarction (MI) is associated with endothelial dysfunction resulting in an imbalance in endothelium-derived vasodilators and vasoconstrictors. We have previously shown that despite increased endothelin (ET) plasma levels, the coronary vasoconstrictor effect of endogenous ET is abolished after MI. In

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