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A3332

Sigma-Aldrich

8-Acetoxypyrene-1,3,6-trisulfonic acid trisodium salt

≥98%

Synonym(s):
1-Acetoxypyrene-3,6,8-trisulfonic acid trisodium salt, Trisodium-8-acetoxypyrene-1,3,6-trisulfonate
Empirical Formula (Hill Notation):
C18H9Na3O11S3
CAS Number:
Molecular Weight:
566.42
Beilstein:
9538267
MDL number:
PubChem Substance ID:

assay

≥98%

mp

238-248 °C (dec.) (lit.)

storage temp.

−20°C

SMILES string

[Na+].[Na+].[Na+].CC(=O)Oc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O

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Application

A membrane permeable, nonfluorescent reagent . This fluorochrome enters the cell cytoplasm, where its acetic group is cleaved by intracellular, nonspecific esterases, which forms the impermeable, fluorescent form of the molecule. Utilized as a phloem-mobile symplasmic tracer having staining characteristics that differ from the commonly used CFDA (5-(6-)carboxyfluorescein diacetate).

Substrates

Esterase substrate

Certificate of Analysis

Certificate of Origin

Photothermal quantitation of nanogram quantities of Coomassie brilliant blue stained proteins in denaturing polyacrylamide gels.
K Peck et al.
Analytical chemistry, 58(13), 2876-2879 (1986-11-01)

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