All Photos(2)

A4043

Sigma-Aldrich

Azure B

certified by the Biological Stain Commission

Synonym(s):
Azure I, Methyleneazure, N,N,N′-Trimethylthionin
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52010
Beilstein:
3922630
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥89%

mp

205-210 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

fluorescence

λex 648 in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

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Application

Azure B has been used for the staining of chromosomes.

Packaging

5, 25 g in glass bottle

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

Suitability

Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Nuclear DNA content, base composition, and cytogenetic characterization of Christia obcordata.
Sakhanokho HG and Islam-Faridi N
J. Am. Soc. Hort. Sci., 138, 205-209 (2013)
Bain BJ, et al.
Dacie and Lewis Practical Haematology (2016)
Cell and Tissue Culture in Forestry (2012)
E Rosales et al.
Environmental science and pollution research international, 19(5), 1738-1746 (2011-12-14)
The aim of this work was to improve the ability of electro-Fenton technique for the remediation of wastewater contaminated with synthetic dyes using a model azo dye such as Azure B. Batch experiments were conducted to study the effects of
Luaine Bandounas et al.
New biotechnology, 30(2), 196-204 (2012-05-09)
In this study we have investigated the molecular background of the previously reported dye decolourization potential of Bacillus sp. LD003. Strain LD003 was previously isolated on Kraft lignin and was able to decolourize various lignin model dyes. Specifically Azure B

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