This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins.
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
recombinant polyHistidine tagged fusion protein.
Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.