Apoptosis-inducing factor (AIF) is encoded by the gene mapped to human chromosome X (Xq25-Xq26). It is a 57 kDa mitochondrial flavoprotein. AIF contains two mitochondrial localization sequences and two putative nuclear localization sequences.
Apoptosis-Inducing Factor (AIF) is a mitochondrial flavoprotein that can induce apoptosis in isolated nuclei. Studies have reported that AIF can induce the realease of cytochrome c and caspase 9. Bcl-2 is known to inhibit AIF release without affecting its apoptotic functions.
Rabbit Anti-AIF antibody recognizes human and mouse AIF (57kDa). Staining of AIF in immunoblotting is specifically inhibited with the AIF immunizing peptide (human, amino acids 593-613).
synthetic peptide corresponding to the C-terminus of human Apoptosis Inducing Factor (AIF), (amino acids 593-613), conjugated to KLH.
Protein lysates from 2CLL, 10 MCl and Jeko-1 cells were analyzed by western blot using anti-AIF as the primary antibody. Cells also underwent intracellular delivery by being transfected with anti-AIF at a final dilution of 1:2500 and then analyzed by FLOW cytometry.
Rabbit Anti-Apoptosis-Inducing Factor (AIF) antibody has been used for immunoblot analysis at 1:1000 and 1:2000 dilutions. The antibody can also be used for microarray applications.
Microinjection of apoptosis-inducing factor (AIF) into the cytoplasm of intact cells induces chromatin condensation, dissipation of the mitochondrial transmembrane potential and exposure of phosphatidylserine in the plasma membrane.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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