A9477

Sigma-Aldrich

Aldosterone

≥95% (HPLC)

Synonym(s):
18-Aldocorticosterone, Reichstein X, 11β,21-Dihydroxy-3,20-dioxo-4-pregnen-18-al, 11β,21-Dihydroxypregn-4-ene-3,18,20-trione
Empirical Formula (Hill Notation):
C21H28O5
CAS Number:
Molecular Weight:
360.44
Beilstein/REAXYS Number:
3224996
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

biological source

synthetic (organic)

sterility

non-sterile

assay

≥95% (HPLC)

form

powder

application(s)

cell culture | mammalian: suitable

solubility

chloroform: 20 mg/mL, clear to slightly hazy, colorless to light yellow

shipped in

ambient

storage temp.

room temp

SMILES string

C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@H](C(=O)CO)[C@]4(C[C@H](O)[C@H]23)C=O

InChI

1S/C21H28O5/c1-20-7-6-13(24)8-12(20)2-3-14-15-4-5-16(18(26)10-22)21(15,11-23)9-17(25)19(14)20/h8,11,14-17,19,22,25H,2-7,9-10H2,1H3/t14-,15-,16+,17-,19+,20-,21+/m0/s1

InChI key

PQSUYGKTWSAVDQ-ZVIOFETBSA-N

Gene Information

human ... SERPINA6(866)
rat ... Ar(24208), Nr3c2(25672)

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Application

Aldosterone has been used:
  • as a RPMI-1640 medium supplement for podocyte culture to test its pathogenicity in obesity-related glomerulopathy (ORG)
  • in combination with salt to induce renal injury in mice
  • to stimulate primary liver sinusoidal endothelial cells (LSECs) and in the generation of hyperaldosteronism model in mice

The d-isomer of aldosterone is considered to be the biologically active isomer.

Packaging

100 mg in poly bottle
5, 25 mg in glass insert

Biochem/physiol Actions

Aldosterone is a biologically active aldosterone isomer; a mineralocorticoid produced by the adrenal cortex. It induces urinary excretion of K+ and renal reabsorption of Na+. It is produced from adrenocorticotropic hormone (ACTH) and is essential for maintaining sodium homeostasis in the distal tubules. Aldosterone binds to the mineralocorticoid receptor and may modulate vascular system and induce kidney damage. It contributes to the progression of chronic kidney disease(CKD). Aldosterone is part of the renin-angiotensin-aldosterone system (RAAS).

Other Notes

Exists as an equilibrium mixture of the aldehyde and the hemiacetal.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product A9477, Aldosterone, the d- or l- isomer?

    The A9477 is the d-Aldosterone (per our supplier), which is considered to be the biologically active isomer.

  6. How do I make a stock solution of Product A9477, Aldosterone?

    To prepare a 100 μg/ml stock solution, add 5 ml of absolute ethanol to 5 mg, gently swirl to dissolve, then add 45 ml sterile medium. Freeze the stock in working aliquots, but avoid repeated freeze/thaw cycles.

  7. Is Product A9477, Aldosterone, tested for solubility?

    Yes, A9477 is tested for solubility using 20mg/ml in chloroform. For biological applications, you can solubilize aldosterone in ethanol to at least 1 mg/mL. Please refer to literature references for further solubility information.

  8. What is the difference between Aldosterone Products A9477 and 05521?

    The main differences between A9477 and 05521 are the assays performed on the products. The assay results are lot specific and can be viewed on the Certificate of Analysis.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Role of bardoxolone methyl, a nuclear factor erythroid 2-related factor 2 activator, in aldosterone-and salt-induced renal injury
Hisamichi M
Hypertension Research, 41(1), 8-8 (2018)
Aldosterone and Mineralocorticoid Receptor System in Cardiovascular Physiology and Pathophysiology
Cannavo A, et al.
Oxidative Medicine and Cellular Longevity, 2018 (2018)
Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration
Luo X, et al.
Redox Biology, 13(5), 508-521 (2017)
Aldosterone and renin measurements
Cartledge S and Lawson N
Annals of Clinical Biochemistry, 37(3), 262-278 (2000)
Anne D Thuesen et al.
American journal of physiology. Renal physiology, 317(2), F254-F263 (2019-05-02)
T-type Ca2+ channel Cav3.1 promotes microvessel contraction ex vivo. It was hypothesized that in vivo, functional deletion of Cav3.1, but not Cav3.2, protects mice against angiotensin II (ANG II)-induced hypertension. Mean arterial blood pressure (MAP) and heart rate were measured...

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