Documents

A9484

Sigma-Aldrich

Anti-AIF1 (72-84) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-Allograft inflammatory factor 1 isoform 1, Anti-IBA1, Anti-IRT-1

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~10 kDa

species reactivity

human

application(s)

western blot: 1:500-1:2,000

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... AIF1(199)

General description

Allograft inflammatory factor-1 (AIF-1) is a vascular smooth muscle cells (VSMC) protein that is induced during allograft and angioplasty injury. Studies have reported that AIF-1 can polymerize actin, stimulate Rac1 and facilitate the migration of VSMCs.
Rabbit Anti-AIF1 (72-84) antibody binds to amino acids 72-84 of human AIF1.

Immunogen

synthetic peptide corresponding to amino acids 72-84 of human AIF1

Application

Rabbit Anti-AIF1 (72-84) antibody can be used for western blot applications at dilutions ranging from 1:500 to 1:2,000.
Immunoflourescence was performed on cyrosectioned mouse retinas using rabbit anti-AIF1 antibody as the primary. Anti-AIF1 labeled photoreceptor segments and the outer plexiform layer.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Elysse M Knight et al.
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Hans G Novrup et al.
Journal of neuroinflammation, 11, 159-159 (2014-09-11)
Glial cell activation and overproduction of inflammatory mediators in the central nervous system (CNS) have been implicated in acute traumatic injuries to the CNS, including spinal cord injury (SCI). Elevated levels of the proinflammatory cytokine tumor necrosis factor (TNF), which...
Michael V Autieri et al.
Circulation research, 92(10), 1107-1114 (2003-04-26)
Development of vascular restenosis is a multifaceted process characterized by migration and proliferation of vascular smooth muscle cells (VSMCs), resulting in loss of lumen diameter. Characterization of proteins that mediate this process is essential in our understanding of the pathogenesis...
Gareth R Howell et al.
Neurobiology of disease, 71, 44-52 (2014-08-19)
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American journal of respiratory cell and molecular biology, 51(1), 155-162 (2014-02-14)
Pulmonary arterial hypertension (PAH) is characterized by adverse remodeling of pulmonary arteries. Although the origin of the disease and its underlying pathophysiology remain incompletely understood, inflammation has been identified as a central mediator of disease progression. Oxidative inflammatory conditions support...

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