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A9655

Sigma-Aldrich

Auramine O

Dye content ≥80 %, certified by the Biological Stain Commission

Synonym(s):
Pyoctaninum aureum, 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2
Empirical Formula (Hill Notation):
C17H21N3 · HCl
CAS Number:
Molecular Weight:
303.83
Colour Index Number:
41000
Beilstein:
4030061
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥80%

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λmax

370 nm in H2O
432 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

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General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

Packaging

25 g in glass bottle

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Equilibrium and kinetic studies on sorption of basic dyes by a natural biopolymer poly (γ-glutamic acid).
Inbaraj B S, et al.
Biochemical Engineering Journal, 31(3), 204-215 (2006)
E W Koneman.
Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 1565-1565 (2006)
Lynne S.
Practical Guide to Diagnostic Parasitology, 486-486 (2009)
Nadav Amdursky et al.
The journal of physical chemistry. B, 116(45), 13389-13395 (2012-10-24)
There is an indispensable need for a fluorescence marker for the detection of amyloid fibrils, where, at present, the most used marker is thioflavin-T (ThT). Here, we present the use of auramine-O (AuO) as a possible alternative to ThT. As
S Wilson et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 14(9), 1164-1168 (2010-09-08)
Direct sputum smear microscopy is usually less sensitive than the indirect approach using concentration by centrifugation, but laboratories often do not have access to appropriate equipment. An alternative method of sample concentration has been developed based on magnetic beads coated

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