The Cultured Cells Acellularization Kit is for ECM preparation to be used as a supportive layer for cell growth, and for other applications like ECM characterization by immunofluorescence labeling or by Western blot analysis. This kit provides all of the reagents required for an easy and efficient removal of ECM-producing cultured cells (acellularization) leaving the underlying ECM intact. Additionally, it contains Ponceau S Solution for ECM staining and Solubilization Buffer for ECM protein extraction for downstream applications.
The kit was qualified with bovine aorta endothelial cells (BAEC), human foreskin fibroblasts, rabbit corneal primary cells, and with the NIH 3T3 cell line. Sufficient reagents are provide to process thirty 10 cm culture dishes.