APOAC

Sigma-Aldrich

Annexin V-Cy3 Apoptosis Detection Kit

NACRES:
NA.32
Pricing and availability is not currently available.

usage

sufficient for 200 tests

Quality Level

packaging

pkg of 1 kit

application(s)

cell analysis: suitable
detection: suitable
flow cytometry: suitable

detection method

fluorometric

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principle

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Legal Information

Cy3 is a trademark of Cytiva

Kit Components Also Available Separately

Product No.
Description
SDS

  • C50416-Carboxyfluorescein diacetate 10 mg

  • Annexin V-Cy3.18 Conjugate from human placenta 10 μg

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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