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Bathocuproinedisulfonic acid disodium salt

for spectrophotometric det. of Cu, Fe

Disodium bathocuproinedisulfonate, 2,9-Dimethyl-4,7-diphenyl-1,10-phenantrolinedisulfonic acid disodium salt
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:


7.2-9.1% dry basis (ICP)

Quality Level




for spectrophotometric det. of Cu, Fe


H2O: 50 mg/mL


suitable for determination of copper


diagnostic assay manufacturing

storage temp.

room temp

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General description

Bathocuproinedisulfonic acid is a strong copper chelator. It is used for the determination of copper and iron in biological samples.


  • Bathocuproinedisulfonic acid disodium salt has been used as a copper chelator for specific assay in Arabidopsis seedlings.
  • It has been used as an electron chain-associated free radical production inhibitor for superoxide dismutase assay in cells.
  • It has been added to the culture media of trypanosomes as a copper chelator for the stabilization of cysteine.

Other Notes

Mixture of isomers

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Shinya Ogawa et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 909, 34-36 (2012-11-17)
HPLC eluent systems employing acetonitrile and methanol were evaluated for the quantitation of glutathione (GSH) and phytochelatin (PC(n)), a family of peptides implicated in heavy-metal detoxification in higher plants. The detection system is based on the dequenching of copper(I)-bathocuproine disulfonate
Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue.
Weydert CJ and Cullen JJ
Nature Protocols, 5, 51-66 (2010)
Shinya Ogawa et al.
Analytical chemistry, 81(21), 9199-9200 (2009-09-30)
Reoptimization of analytical conditions was performed for a high-performance liquid chromatographic (HPLC) detection system for Cu(I) chelators based on the dequenching of Cu(I)-bathocuproine disulfonate complexes that occurs in the presence of Cu(I) chelators. The revision corrects for emission and excitation
Kristine Hardy et al.
Free radical biology & medicine, 37(10), 1550-1563 (2004-10-13)
Antioxidants can inhibit the proliferation of T lymphocytes induced by mitogens. This has been postulated to be due to their scavenging of reactive oxygen species which may act as second messengers in the antigen-induced signaling cascade leading to cell proliferation.
Vicente Sancenón et al.
The Journal of biological chemistry, 279(15), 15348-15355 (2004-01-17)
Copper plays a dual role in aerobic organisms, as both an essential and a potentially toxic element. To ensure copper availability while avoiding its toxic effects, organisms have developed complex homeostatic networks to control copper uptake, distribution, and utilization. In

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