B3269

Sigma-Aldrich

Bromphenol Blue–Xylene Cyanole Dye solution

lyophilized powder

NACRES:
NA.47
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

application(s)

hematology: suitable
histology: suitable

pH range

8 - 8.6

solubility

water: 0.2 vial/mL, blue

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

General description

Bromophenol Blue–Xylene Cyanol Dye solution is a tracking dye for nucleic acid electrophoresis in agarose or polyacrylamide gels including DNA sequencing.

Application

Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.

Reconstitution

Add 5 mL water to the vial to dissolve. Reconstituted solution contains 0.5% bromphenol blue and 0.5% xylene cyanole. Dilute 1:10 for nucleic acid electrophoresis in agarose or polyacrylamide gels or add 4 parts formamide to 1 part dye solution for DNA sequencing.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes

T

Risk Statement

60-61-36/37/38

Safety Statement

53-26-45

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
CLIP: construction of cDNA libraries for high-throughput sequencing from RNAs cross-linked to proteins in vivo.
Wang Z, et al.
Methods, 48, 287-293 (2009)
Genomic sequencing.
Church GM and Walter G
Proceedings of the National Academy of Sciences of the USA, 81.7, 1991-1995 (1984)

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