B6185

Sigma-Aldrich

Bionic Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Synonym(s):
Sodium Borate Electrophoresis Buffer
NACRES:
NA.25
Pricing and availability is not currently available.

Quality Level

grade

for molecular biology

form

solution

concentration

10 ×

impurities

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

storage temp.

room temp

Related Categories

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Application

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Features and Benefits

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Caution

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Carmen Naturil-Alfonso et al.
PloS one, 7(12), e51271-e51271 (2012-12-20)
Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate...
Maria Desemparats Saenz-de-Juano et al.
Biology of reproduction, 87(4), 91-91 (2012-08-03)
Studies of embryo cryopreservation efficiency have focused mainly on technical and embryo factors. To determine how a slow freezing process affects embryo and fetal development, we studied in vivo development ability after the freezing procedure by assessing blastocyst development at...
Fast short-fragment PCR for rapid and sensitive detection of shrimp viruses.
Mrotzek G, et al.
Journal of Virological Methods, 168, 262-266 (2010)
Protocols
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Read More

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