B9643

Supelco

Bicinchoninic Acid solution

Synonym(s):
BCA
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

suitability

suitable for determination of protein concentration

General description

Protein determination is one of the most common operations performed in biochemical research. Bicinchoninic acid solution is used in the bicinchoninic acid (BCA) assay, which is equivalent to the Lowry procedure. Both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. The BCA assay is more sensitive and applicable than either biuret or Lowry procedures.

Application

Bicinchoninic Acid solution (Bicinchoninic acid protein assay kit) is used :
  • For the determination of total protein content in the process of quantifying bromelain, extracted from the pineapple stem residues using aqueous micellar two-phase systems composed of ionic liquids as co-surfactants.
  • For the determination of bovine serum albumin (BSA) in biomimetic calcium phosphate coating co-precipitated on titanium alloy plates.

Packaging

1 L in poly bottle
Also available as part of the Bicinchoninic Acid Kit for Protein Determination (BCA-1).

Features and Benefits

  • It is easy to use.
  • The color complex is stable.
  • There is less susceptibility to detergents.
  • It is applicable over a broad range of protein concentrations.

Suitability

This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Shabnam Khatibi et al.
BMC systems biology, 11(1), 48-48 (2017-04-15)
Transforming growth factor β (TGF-β) signalling regulates the development of embryos and tissue homeostasis in adults. In conjunction with other oncogenic changes, long-term perturbation of TGF-β signalling is associated with cancer metastasis. Although TGF-β signalling can be complex, many of...
Zhenhua Ma et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 216, 29-37 (2018-11-12)
This study explored the impact of fishmeal replacement by commercial soybean meal (SM) and EnzoMeal (EZM) on Asian seabass Lates calcarifer growth performance using six diets. The six diets comprised two sources of plant proteins with three levels each, including...
Shabnam Khatibi et al.
Growth factors (Chur, Switzerland), 35(2-3), 100-124 (2017-09-28)
Mathematical models for TGF-β and IL-6 signalling have been linked, providing a platform for analyzing the crosstalk between the systems. An integrated IL-6:TGF-β model was developed via a reduced set of reaction equations which incorporate both feedback loops and appropriate...
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente AF, et al.
Process Biochemistry (Oxford, United Kingdom), 51(4), 528-534 (2016)
Diego García-Ayuso et al.
Experimental eye research, 161, 10-16 (2017-05-30)
In this work we study the effects of an acute light-induced retinal degeneration on the population of melanopsin positive retinal ganglion cells (m
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