BCA1

Sigma-Aldrich

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

Synonym(s):
BCA protein assay
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

usage

sufficient for ~500 assays

storage temp.

2-8°C

Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.

Features and Benefits

  • Simple, sensitive colorimetric assay for proteins
  • Two stable reagents are used for the working solution
  • Faster and easier than the Lowry protein assay
  • Compatible with many detergents both ionic and nonionic
  • Less variation between different proteins than the Bradford dye-binding assay
  • Adaptable for use in microwell plates

Principle

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Kit Components Only

Product No.
Description

  • Bicinchoninic Acid Solution 1 L

  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL

  • Protein Standard Solution 5 x 1

Pictograms

Environment

Signal Word

Warning

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes

N

Risk Statement

50/53

Safety Statement

61

RIDADR

UN 3082 9 / PGIII

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Salivary proteome patterns of individuals exposed to High Altitude
Jain S, et al.
Archives of Oral Biology, 96, 104-112 (2018)
TGFB1 modulates in vitro secretory activity and viability of equine luteal cells
Galvao A, et al.
Cytokine, 110, 316-327 (2018)
Human OATP1B1 (SLCO1B1) transports sulfated bile acids and bile salts with particular efficiency
Toth B, et al.
Toxicology in vitro, 52, 189-194 (2018)
R E Brown et al.
Analytical biochemistry, 180(1), 136-139 (1989-07-01)
Protein quantitation based on bicinchoninic acid (BCA) is simple, sensitive, and tolerant to many detergents and substances known to interfere with the Lowry method. However, certain compounds often used during protein purification do interfere with the BCA protein assay. The...
K J Wiechelman et al.
Analytical biochemistry, 175(1), 231-237 (1988-11-15)
The colored complex formed between Cu+ and bicinchoninic acid is the basis of the bicinchoninic acid protein assay (P. K. Smith, R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N....
Related Content
A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.
Read More

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