A solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal capture antibody specific for the NH2 terminus of hAβ has been coated onto the multiwell strips. hAβ antigen in standards and samples binds to the capture antibody. A detection antibody specific for the hAβ 1−40 or 1−42 sequence binds the immobilized hAβ. An anti-rabbit IgG-HRP completes the sandwich. The reaction is visualized by TMB substrate, followed by the stop solution. The intensity of the yellow color is directly proportional to the concentration of hAβ in the original sample.
The ELISA can be used for in vitro quantitative determination of human native and recombinant β-amyloid 1-42 (hAβ42) protein in serum, cell culture supernatants and other biological samples. The Aβ42 antibody used in this kit selectively detects hAβ42 and not hAβ40.
Human β-amyloid (hAβ) ELISAs are designed for in vitro quantitative detection of native and recombinant β-amyloid in sera, cell culture supernatants and other biological samples. hAβ′s are main component of the protein deposits in neurofibrillary tangles, neuritic plaques and neuropil threads in the cerebral cortex of Alzheimer′s Disease (AD) and Down′s Syndrome. The antibodies used in these kits selectively differentiate between Aβ40 and Aβ42/Aβ43.