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C0297

Sigma-Aldrich

Monoclonal Anti-Caldesmon antibody produced in mouse

clone C21, ascites fluid

MDL number:

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

C21, monoclonal

contains

15 mM sodium azide

species reactivity

mammals

application(s)

indirect ELISA: 1:2,500
western blot: suitable

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CALD1(800)
mouse ... Cald1(109624)
rat ... Cald1(25687)

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Specificity

The antibody is directed against an epitope near the carboxy-terminus, Ca2+/calmodulin and F-actin binding site of caldesmon. Using ELISA, the antibody is able to inhibit caldesmon binding to Ca2+/calmodulin and F-actin. By immunoblotting, the antibody shows broad species reactivity to non-muscle caldesmon of mammalian cells.

Immunogen

chicken caldesmon

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Erik Y Zhang et al.
The American journal of pathology, 164(2), 601-612 (2004-01-27)
Partial bladder outlet obstruction (PBOO) induces remodeling of urinary bladder smooth muscle (detrusor). We demonstrate an increase in bladder wall mass, muscle bundle size, and a threefold increase in the cross-sectional area of detrusor myocytes following PBOO in male New...
J J Lin et al.
Hybridoma, 7(3), 273-288 (1988-06-01)
Monoclonal antibodies, C2, C9, C18, C21 and C23, against chicken gizzard caldesmon have been prepared and characterized. These antibodies reacted with gizzard caldesmon (150 KDa) by enzyme-linked immunosorbent assay and protein immunoblotting. Immunofluorescence microscopy with these antibodies on cultured gizzard...
Julie L Boerner et al.
Oncogene, 22(43), 6679-6689 (2003-10-14)
Mutations within members of the EGF/ErbB receptor family frequently release the oncogenic potential of these receptors, resulting in the activation of downstream signaling events independent of ligand regulatory constraints. We previously have demonstrated that the signal transduction events originating from...
Ali Hashemi Gheinani et al.
The Journal of biological chemistry, 290(11), 7067-7086 (2015-01-18)
MicroRNA miR-199a-5p impairs tight junction formation, leading to increased urothelial permeability in bladder pain syndrome. Now, using transcriptome analysis in urothelial TEU-2 cells, we implicate it in the regulation of cell cycle, cytoskeleton remodeling, TGF, and WNT signaling pathways. MiR-199a-5p...
M J McManus et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(21), 11351-11356 (1997-10-23)
The avian erythroblastosis viral oncogene (v-erbB) encodes a receptor tyrosine kinase that possesses sarcomagenic and leukemogenic potential. We have expressed transforming and nontransforming mutants of v-erbB in fibroblasts to detect transformation-associated signal transduction events. Coimmunoprecipitation and affinity chromatography have been...

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