Documents

C3997

Sigma-Aldrich

Anti-CAPZB (71-85) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-CAPZ, Anti-CAPPB, Anti-F-actin-capping protein subunit β isoform 2, Anti-CAPB

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~31 kDa

species reactivity

human

application(s)

western blot: 1:500-1:2,000

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CAPZB(832)

General description

The gene CAPZB (F-actin-capping protein subunit β) is mapped to human chromosome 1p36.1. The protein co-localizes with the actin filaments.

Immunogen

synthetic peptide corresponding to amino acids 71-85 of human CAPZB

Application

Anti-CAPZB (71-85) antibody produced in rabbit is suitable for western blot at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

CAPZB (F-actin-capping protein subunit β) are F-actin-capping proteins. Absence of CAPZB reduces actin filament depolymerization and capping. CAPZB also plays a role in the regulation of cell morphology. T cell surface protein CD2 regulates T cell signalling partly by associating with CAPZB, CD2AP (CD2-associated protein) and CIN85 (SH3 domain-containing kinase-binding protein 1).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Certificate of Analysis

Certificate of Origin

S A Bustin et al.
DNA and cell biology, 20(6), 331-338 (2001-07-11)
The role of ion channels in carcinogenesis and tumor progression remains unclear. We have used suppression subtractive hybridization of mRNA from paired normal colon epithelium and tumor, followed by quantitative kinetic RT-PCR, to demonstrate that the transcription of two members...
Nicholas J Hutchings et al.
The Journal of biological chemistry, 278(25), 22396-22403 (2003-04-12)
Recruitment of CD2 to the immunological synapse in response to antigen is dependent on its proline-rich cytoplasmic tail. A peptide from this region (CD2:322-339) isolated CMS (human CD2AP); a related protein, CIN85; and the actin capping protein, CAPZ from a...
E A Barron-Casella et al.
The Journal of biological chemistry, 270(37), 21472-21479 (1995-09-15)
From a human retinal cDNA library, we have isolated cDNAs that are homologs for the alpha 2 and beta subunits of chicken Cap Z. The derived human alpha subunit shares 95% amino acid identity with the chicken alpha 2 subunit;...
Siau Wei Bai et al.
BMC biology, 9, 54-54 (2011-08-13)
Cell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways. Here we use data from a genome-wide RNAi morphology screen in Drosophila melanogaster cells...

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