All Photos(3)

C6244

Sigma-Aldrich

Anti-CAMKK2 (7-21) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-Calcium/calmodulin-dependent protein kinase kinase 2 β isoform 1 (Homo sapiens), Anti-CAMKK, Anti-CAMKKB
MDL number:

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~64 kDa

species reactivity

human

application(s)

western blot: 1:500-1:2,000

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CAMKK2(10645)

Immunogen

synthetic peptide corresponding to amino acids 7-21 of human CAMKK2.

Application

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if. Anti-CAMKK2 (7-21) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:2,000.

Biochem/physiol Actions

Calcium/calmodulin-dependent protein kinase kinase 2 is an enzyme encoded by the CAMKK2 gene in humans. CaMKK gene is located on human chromosome 12. It is also referred as CAMKK and CAMKKB. CAMKKB pathway is involved in the activation of AMP-activated protein kinase (AMPK) by baicalin and acts as an upstream kinase of AMPK in response to baicalin. Calmodulin (CaM) is used in many cellular Ca2+-dependent signaling cascades as intracellular Ca2+ receptor. It binds and activates enzyme CaM kinases (CaMKs). CaMKK2 is one of the most versatile among the calmodulin kinases. It phosphorylates and activates CaMKI, CaMKIV, and AMP-activated protein kinase. CAMKK plays an important role during human cytomegalovirus (HCMV)-mediated glycolytic activation and HCMV replication.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Jessica McArdle et al.
Journal of virology, 85(2), 705-714 (2010-11-19)
Viruses depend on the host cell to provide the energy and biomolecular subunits necessary for production of viral progeny. We have previously reported that human cytomegalovirus (HCMV) infection induces dramatic changes to central carbon metabolism, including glycolysis, the tricarboxylic acid...
Ying Ma et al.
PloS one, 7(10), e47900-e47900 (2012-10-31)
AMP-activated protein kinase (AMPK) plays an important role in mediating energy metabolism and is controlled mainly by two upstream kinases, LKB1 or Ca(2+)/calmodulin-dependent protein kinase kinase-β (CaMKKβ). Previously, we found that baicalin, one of the major flavonoids in a traditional...
Luigi Racioppi et al.
The Journal of biological chemistry, 287(38), 31658-31665 (2012-07-11)
Many cellular Ca(2+)-dependent signaling cascades utilize calmodulin (CaM) as the intracellular Ca(2+) receptor. Ca(2+)/CaM binds and activates a plethora of enzymes, including CaM kinases (CaMKs). CaMKK2 is one of the most versatile of the CaMKs and will phosphorylate and activate...

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