C6366

SAFC

EX-CELL® CHO Cloning Medium

Animal-component free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Pricing and availability is not currently available.

Quality Level

sterility

sterile-filtered

form

liquid

storage condition

protect from light

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Application

This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum.

Features and Benefits

Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.

Other Notes

Proprietary formulation containing inorganic salts, sodium bicarbonate, essential and non-essential amino acids, vitamins, trace elements, plant hydrolysates, and other organic compounds.
Does not contain antibiotics, antimycotics, L-glutamine, insulin, or transferrin.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Reconstitution

Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Articles
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
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The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).
Read More
In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.
Read More
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
Read More

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