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C9491

Sigma-Aldrich

Anti-CtBP1, N-terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-Carboxy-terminal binding protein 1
MDL number:

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

human, mouse

technique(s)

immunoprecipitation (IP): 5-10 μL using lysates of K562 cell line
microarray: suitable
western blot: 1:250-1:500 using mouse brain lysates
western blot: 1:500-1:1,000 using K562 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CTBP1(1487)
mouse ... Ctbp1(13016)

Immunogen

synthetic peptide corresponding amino acids 2-18 of human CtBP1. This sequence is conserved in mouse and does not cross-react with CtBP2.

Application

Anti-CtBP1, N-terminal antibody produced in rabbit is suitable for immunoprecipitation and immunoblotting at a working dilution of 1:500-1:1000 using cell lysates of the K562 cell line and 1:250-1:500 using mouse brain lysates.

Biochem/physiol Actions

Apart from E1A protein, CtBP1 interacts with a wide variety of cellular factors such as DNA-binding transcription factors that participate in several developmental biological pathways and processes by binding to the carboxy terminal. It also interacts with polycomb group proteins and thus a component of the chromatin remodeling machinery and transcriptional repression. Pnn/DRS, a phosphoprotein involved in the regulation of cell adhesion and modulation of the activity of multiple tumor supressor genes, interacts with CtBP1 and may modulate repression of E-cadherin transcription. CtBP1 associates with SMAD6 and represses BMP signaling of the TGF-β pathway in the nucleus. It acts as transcriptional coregulator in histone acetyltransferase or histone deacetylase (HDAC)-dependent and independent manner.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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