CASP3C

Sigma-Aldrich

Caspase 3 Assay Kit, Colorimetric

NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
sufficient for 100 standard tests (1 ml)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Application

The assay can be performed in 1 mL volume and measured using a spectrophotometer or in 100 ìl volume in a 96 well plate using an ELISA reader.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Other Notes

Use 17 megohm water only

Kit Components Only

Product No.
Description

  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • A0835Ac-DEVD-CHO inhibitor .5 mg

  • A2559Ac-DEVD-pNA substrate 15 mg

  • p-Nitroaniline standard 1 mg

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Codes

T

Risk Statement

23/24/25-33-52/53

Safety Statement

36/37-45-61

RIDADR

UN 3316 9

Flash Point(F)

415.4 °F - closed cup

Flash Point(C)

213 °C - closed cup

Certificate of Analysis
Certificate of Origin
S C Masters et al.
The Journal of biological chemistry, 276(48), 45193-45200 (2001-09-29)
The 14-3-3 proteins are a family of highly conserved eukaryotic regulatory molecules that play important roles in many biological processes including cell cycle control and regulation of cell death. They are able to carry out these effects through binding and...
H Schroeter et al.
The Biochemical journal, 358(Pt 3), 547-557 (2001-09-06)
Oxidative stress has been associated with neuronal loss in neurodegenerative diseases and during age-associated cognitive decline. Flavonoids have been proposed to play a useful role in protecting the central nervous system against oxidative and excitotoxic stress, although the mechanism of...
Wafa A Al-Megrin et al.
Frontiers in physiology, 11, 64-64 (2020-03-03)
The kidney is among the metabolic organs most susceptible to injury, particularly following exposure to xenobiotics and heavy metals. We aimed to explore the potential protective impacts of coenzyme Q10 (CoQ10) on lead acetate (PbAc)-induced nephrotoxicity in rats. Four experimental...
Rafa S Almeer et al.
Environmental science and pollution research international, 27(3), 3401-3412 (2019-12-17)
Mercury (Hg) is a heavy metal toxicant, causing several adverse reactions to animals and humans including reproductive dysfunction. The potential protective role of Ziziphus spina-christi leaf extract (ZSCLE) against testicular impairments associated with mercury chloride (HgCl2) exposure in rats was...
Afrah F Alkhuriji et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-03)
Listeria monocytogenes is a psychrophilic bacterium, which causes widespread zoonosis in the natural environment, and mainly affects goat, sheep, and cattle herds. Recently, we predicted that it can be transmitted through food. It causes listeriosis, a severe infectious disease, which...
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