CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

EC Number:
NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Kit Components Only

Product No.
Description

  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • A1086Ac-DEVD-AMC substrate 2.5 mg

  • A0835Ac-DEVD-CHO inhibitor .5 mg

  • A98917-Amino-4-methylcoumarin standard 1 mg

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Target Organs

Respiratory system

Hazard Codes

Xi

Risk Statement

36/37/38

Safety Statement

26-36

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
Cisplatin is a potent and widely used chemotherapeutic agent. Nephrotoxicity induced by this drug has been well documented. However, very little information is available on cisplatin-induced hepatotoxicity and its underlying mechanism remains unclear. High doses of cisplatin have been known...
V A Smith et al.
The British journal of ophthalmology, 88(5), 619-625 (2004-04-20)
Doxycycline is a broad spectrum antibiotic that chelates metal ions and is frequently used as part of the treatment of ocular surface diseases. Its therapeutic value has been ascribed to an ability to inhibit matrix metalloproteinase (MMP) activity and both...
Song Zhou et al.
International journal of molecular medicine, 45(5), 1305-1316 (2020-04-24)
Oxidized low‑density lipoprotein (ox‑LDL)‑induced vascular endothelial damage, oxidative stress and inflammation play a vital role in the pathophysiology of atherosclerosis. Geniposide is the primary active ingredient from Gardenia jasminoides Ellis associated with anti‑oxidative properties and cardioprotective action. However, the therapeutic...
Radharaman Ray et al.
Drug and chemical toxicology, 28(1), 105-116 (2005-02-22)
Sulfur mustard [SM; bis-(2-chloroethyl) sulfide], which causes skin blistering or vesication [(1991). Histo- and cytopathology of acute epithelial lesions. In: Papirmeister, B., Feister, A. J., Robinson, S. I., Ford, R. D., eds. Medical Defense Against Mustard Gas: Toxic Mechanisms and...
Zinc octa-n-alkyl phthalocyanines in photodynamic therapy: photophysical properties, accumulation and apoptosis in cell cultures, studies in erythrocytes and topical application to Balb/c mice skin
Kaestner,L et al.
Photochemistry and Photobiology, 2, 660-667 (2003)
Articles
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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