Documents

CASP6F

Sigma-Aldrich

Caspase 6 Assay Kit, Fluorimetric

Pricing and availability is not currently available.

shipped in

dry ice

storage temp.

−20°C

General description

The Caspase 6 Fluorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Val-Glu-Ile-Asp-7-amino-4-methyl coumarin (Ac-VEID-AMC) by caspase 6 that results in the release of a 7-amino-4-methyl coumarin (AMC) moiety. The excitation and emission wavelengths of AMC are 360 nm and 440 nm, respectively. The concentration of the AMC released can be calculated from a calibration curve prepared from AMC standards (included with the kit).
The kit detects caspase 6 activity in crude and purified preparations of caspase 6.
Complete kit contains:
  • purified caspase 6 for a positive control
  • caspase 6 inhibitor
  • AMC fluorescent standard

Quantity

The kit provides sufficient reagents for 100 tests of 100 μl in 96-well plates.

Kit Components Only

Product No.
Description

  • Acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin Substrate .25 mL

  • 7-Amino-4-methylcoumarin Standard 1 mL

  • Caspase-6 5 μg

Hazard Codes

Xn

Risk Statement

22-36/37/38

Safety Statement

23-26-36

Certificate of Analysis
Certificate of Origin
P Villa et al.
Trends in biochemical sciences, 22(10), 388-393 (1997-11-14)
Five years ago, little was known about mechanisms of apoptotic execution. Now, one class of cell-death gene, the cysteine and aspartases (caspases) has come under intensive study. This review discusses the two classes of caspases, the reasons why humans may...

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