CASP8C

Sigma-Aldrich

Caspase 8 Assay Kit, Colorimetric

NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP8(841)

General description

Caspases (cysteine-requiring aspartate proteases) belong to a highly conserved family of cysteine proteases with specificity for aspartic acid residues found in their substrates. Caspases play a central role in apoptosis. Inhibition of caspases prevents the cell death process. Currently, there are 13 caspases recognized in mammals. Caspases are classified as initiators and effectors. Caspase 8 belongs to the class of initiators. It possesses a prodomain at the terminal and contains the death effector domain which serves as protein-protein interaction motif for caspase 8. With respect to substrate specificity, caspase 8 is classified as a member of group III. It specifically recognizes the (I/L/V) EXD sequence on the substrate.

Application

The Caspase 8 Colorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-p-Nitroaniline (Ac-IETD-pNA) by caspase-8 that results in the release of a p-Nitroaniline (p-NA) moiety. The p-NA is read at 405 nm. The concentration of the p-NA released can be calculated from a calibration curve prepared from p-NA standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
  • purified caspase 8 for a positive control
  • caspase 8 inhibitor
  • p-NA fluorescent standard

Quantity

The kit provides sufficient reagents for 100 tests of 100 μL in 96-well plates.

Kit Components Only

Product No.
Description

  • Acetyl-Ile-Glu-Thr-Asp-p-nitroaniline Substrate 100 μL

  • 10X Assay Buffer 5 mL

  • 5X Lysis Buffer 5 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • Acetyl-Ile-Glu-Thr-Asp-Aldehyde Inhibitor 100 μL

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Febrile-Range Hyperthermia Accelerates Caspase-Dependent Apoptosis in Human Neutrophils
Nagarsekar A, et al.
Journal of Immunology, 181, 2638-2638 (2018)
Modulation of DNA damage response and induction of apoptosis mediates synergism between doxorubicin and a new imidazopyridine derivative in breast and lung cancer cells
El-Awady RA, et al.
DNA Repair, 37, 1-11 (2016)
Basic Concepts of Molecular Pathology, 32-32 (2009)
RNAi-Mediated Simultaneous Downregulation of uPAR and Cathepsin B Induces Caspase 8-Mediated Apoptosis in SNB19 Human Glioma Cells
Gondi CS, et al.
Molecular Cancer Therapeutics, 5(12), 3197-3197 (2006)
Satyam Kumar Agrawal et al.
Nutrition and cancer, 63(5), 802-813 (2011-06-30)
In this study, the apoptosis-inducing effect of an alcoholic extract from Erythrina suberosa stem bark (ESB) was investigated using human promyelocytic leukemia HL60 cells. Cell viability was estimated by MTT assay. We found that the ESB inhibited cell proliferation in...

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