CASP8F

Sigma-Aldrich

Caspase 8 Assay Kit, Fluorimetric

Pricing and availability is not currently available.

usage

sufficient for 40 standard tests (2 ml)
sufficient for 800 multiwell tests (100 μl in 96-well plates)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP8(841)

General description

The Caspase 8 Fluorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-7-amino-4-methyl coumarin (Ac-IETD-AMC) by caspase-8 that results in the release of a 7-amino-4-methyl coumarin (AMC) moiety. The excitation and emission wavelengths of AMC are 360 nm and 460 nm, respectively. The concentration of the AMC released can be calculated from a calibration curve prepared from AMC standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
  • purified caspase 8 for a positive control
  • caspase 8 inhibitor
  • AMC fluorescent standard

Kit Components Only

Product No.
Description

  • Acetyl-Ile-Glu-Thr-Asp-7-amido-4-methylcoumarin Substrate 1 mL

  • 7-Amino-4-methylcoumarin Standard 1 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • Acetyl-Ile-Glu-Thr-Asp-Aldehyde Inhibitor .5 mL

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Codes

T

Risk Statement

61

Safety Statement

53-45

Flash Point(F)

185 °F

Flash Point(C)

85 °C

Certificate of Analysis
Certificate of Origin
H R Stennicke et al.
The Journal of biological chemistry, 272(41), 25719-25723 (1997-11-05)
The observation that the nematode cell death effector gene product Ced-3 is homologous to human interleukin-1beta-converting enzyme (caspase-1) has led to the discovery of at least nine other human caspases, many of which are implicated as mediators of apoptosis. Significant...
P Villa et al.
Trends in biochemical sciences, 22(10), 388-393 (1997-11-14)
Five years ago, little was known about mechanisms of apoptotic execution. Now, one class of cell-death gene, the cysteine and aspartases (caspases) has come under intensive study. This review discusses the two classes of caspases, the reasons why humans may...

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