CELLYTPN1

Sigma-Aldrich

CelLytic PN Isolation/Extraction Kit

For plant leaves

NACRES:
NA.56
Pricing and availability is not currently available.

Quality Level

usage

 kit sufficient for 30 extractions (20 g of fresh or frozen leaves)

shipped in

wet ice

storage temp.

2-8°C

General description

CelLytic-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones.

Application

The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis.

Other Notes

This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

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Warning

Hazard Statements

Precautionary Statements

RIDADR

UN 3077 9 / PGIII

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Irfan Ullah Khan et al.
Frontiers in plant science, 11, 623-623 (2020-06-13)
Drought stress adversely affects plant growth and development and significantly reduces crop productivity and yields. The phytohormone abscisic acid (ABA) rapidly accumulates in response to drought stress and mediates the expression of stress-responsive genes that help the plant to survive...
Top-down mass spectrometry of histone modifications in sorghum reveals potential epigenetic markers for drought acclimation.
Zhou, et al.
Methods, 184, 29-39 (2020)
Guoyin Liu et al.
Pathogens (Basel, Switzerland), 8(4) (2019-11-30)
WRKYs play important roles in plant development and stress responses. Although MaWRKYs have been comprehensively identified in the banana (Musa acuminata), their in vivo roles and direct targets remain elusive. In this study, a transcript profile analysis indicated the common...
Esther Yakir et al.
Plant physiology, 150(2), 844-857 (2009-04-03)
As an adaptation to life in a world with predictable daily changes, most eukaryotes and some prokaryotes have endogenous circadian (approximately 24 h) clocks. In plants, the circadian clock regulates a diverse range of cellular and physiological events from gene...

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