MilliporeSigma
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CELLYTPN1

Sigma-Aldrich

CelLytic PN Isolation/Extraction Kit

For plant leaves

NACRES:
NA.56

Quality Level

usage

1 kit sufficient for 30 extractions (20 g of fresh or frozen leaves)

shipped in

wet ice

storage temp.

2-8°C

General description

CelLytic-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones.

Application

The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis.

Other Notes

This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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