CLL1033

Sigma-Aldrich

U2OS GFP-LMNB1

NACRES:
NA.81
Pricing and availability is not currently available.

Quality Level

biological source

human female bone (Source Disease: Osteosarcoma)

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... lamin B1(4001)

General description

This product was derived from ATCC Cat. No. HTB-96.
LMNB1 (lamin B1) is a B type lamin protein. LMNB1 is abundantly expressed in various cell types. This gene is located on human chromosome 5q23. The nuclear lamina, an intermediate filament meshwork, is made of LmnB1, LmnB2, A type (LmnA/C) and associated inner nuclear membrane proteins.
U2OS GFP-LMNB1 are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.

Application

To learn more, please visit the Cellular Reporter Cell Line webpage
This product is a human U2OS cell line in which the genomic LaminB1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of LaminB1. Fluorescence imaging shows the characteristic LaminB1 nuclear expression. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.

Biochem/physiol Actions

Nuclear lamin B1 encoded by LMNB1 gene is an intermediate filament protein of the nuclear envelope. The protein has transcriptional co-regulatory activity. It plays a key role in DNA replication, cellular aging and stress responses. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC) patients. Thus, LMNB1 can be used as a potential biomarker for HCC.
Lamin B1 (LMNB1) is essential for proper chromosome condensation in interphase nuclei. It also participates in the distribution of heterochromatin and modulation of gene expression and splicing. Mutations in LMNB1 (lamin B1) gene results in the adult onset demyelinating disease autosomal dominant leukodystrophy (ADLD).

Features and Benefits

The U2OS cells are adherent, with a doubling time of approx. 29 hours.
GFP-tagged on chromosome 5q23.2

Components

U2OS GFP-LMNB1 cells

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Preparation Note

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:6

The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164).

Legal Information

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
The role of lamin B1 for the maintenance of nuclear structure and function.
Camps J, et al.
Nucleus (Austin, Tex.), 6(1), 8-14 (2015)
Nuclear envelope rupture is induced by actin-based nucleus confinement.
Hatch EM and Hetzer MW
The Journal of Cell Biology, 215(1), 27?36-27?36 (2016)
Defects of lipid synthesis are linked to the age-dependent demyelination caused by lamin B1 overexpression.
Rolyan H, et al.
The Journal of Neuroscience, 35(34), 12002-12017 (2015)
Circulating Lamin B1 (LMNB1) Biomarker Detects Early Stages of Liver Cancer in Patients
Sun S, et al.
Journal of Proteome Research, 9(1), 70-78 (2009)
Structural Organization of the Human Gene (LMNB1) Encoding Nuclear Lamin B1
Lin F, et al.
Genomics, 27(2), 230-236 (1995)

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