This product was derived from ATCC Cat. No. HTB-96.
U2OS GFP-HMGA1 are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.
This product is a human U2OS cell line in which the genomic HMGA1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the C-terminus of HMGA1. Fluorescence imaging shows characteristic HMGA1 nuclear expression. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
Features and Benefits
GFP-tagged on chromosome 6p21.
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
U2OS GFP-HMGA1 cells
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
CompoZr is a registered trademark of Sigma-Aldrich Co. LLC