CLL1139

Sigma-Aldrich

SKOV3 Cells GFP-STAT3

NACRES:
NA.81
Pricing and availability is not currently available.

biological source

human female ovary (Source Disease: Ovarian adenocarcinoma)

Quality Level

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... STAT3(6774)

General description

SKOV3 GFP-STAT3 are ovarian adenocarcinoma cells from a human 64 year old caucasion female having a ZFN modification creating a GFP-STAT3 transgene expressed from the endogenous STAT3 gene locus.

This cell line was derived from ATCC Catalog No. HTB-77.

Application

This product is a human SKOV3 cell line in which the genomic STAT3 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® zinc finger nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of STAT3. Fluorescence imaging shows GFP-STAT3 expression throughout the cell. Upon activation with the ligand IL-6, the cell line shows redistribution of STAT3 from the cytoplasm to the nucleus, making it useful for high content screening of compounds that modulate STAT3 activity. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.

To learn more, please visit the
Cellular Reporter Cell Line webpage

Features and Benefits

Zinc Finger Nuclease (ZFN)-mediated targeted integration of a fluorescent GFP tag just behind the start codon of the STAT3 gene on chromosome 17q21.2 to create a cell line exhibiting stable expression of the transgene tagged with GFP on the N-terminus of the protein.

These SKOV3 cells are adherent with a doubling time of approx. 48 hours.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Preparation Note

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:6

The base medium for this cell line is McCoy′s 5A Medium, Cat. No. M8403. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F2442, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 1.5 mM.

Cell freezing medium-DMSO 1X, Cat. No. C6164.

Legal Information

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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