This product corresponds to ATCC Cat. No. CCL-231
SW48 Cells BAX -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.
Media Renewal changes one to two times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
Subculture Ratio: approx. 1:2 to 1:6
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Features and Benefits
Zinc finger nuclease (ZFN) knock out on chromosome 19q13.3-13.4
These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.