CLLS1006

Sigma-Aldrich

SW48 Cells BAX -/-

NACRES:
NA.81
Pricing and availability is not currently available.

Quality Level

biological source

human female colon (Source Disease: Colorectal adenocarcinoma)

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... BAX(581)

General description

This product corresponds to ATCC Cat. No. CCL-231
SW48 Cells BAX -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.

Application

Media Renewal changes one to two times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
Subculture Ratio: approx. 1:2 to 1:6
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Features and Benefits

Zinc finger nuclease (ZFN) knock out on chromosome 19q13.3-13.4
These SW48 cells are adherent, with a doubling time of approx. 35 hours.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Kit Components Also Available Separately

Product No.
Description
SDS

  • SW48 Cells BAX -/- 1 vial

  • SW48 Control Cells 1 vial

Hazard Codes

B

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
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