Documents

CLLS1009

Sigma-Aldrich

SW48 Cells PTEN -/-

NACRES:
NA.81
Pricing and availability is not currently available.

Quality Level

biological source

human female colon (Source Disease: Colorectal adenocarcinoma)

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... PTEN(5728)

General description

SW48 Cells PTEN -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.

Application

Media Renewal changes one to two times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
Subculture Ratio: approx. 1:2 to 1:6
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Features and Benefits

These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 10q23.3

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Kit Components Also Available Separately

Product No.
Description
SDS

  • SW48 Cells PTEN -/- 1 vial

  • SW48 Control Cells 1 vial

Hazard Codes

B

WGK Germany

3

Certificate of Analysis
Certificate of Origin
Related Content
We have applied our revolutionary CompoZr Zinc Finger Nuclease technology to create an unparalleled range of genetically modified mammalian cell lines for use in areas, such as target validation, drug discovery and drug development.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.