Media Renewal changes two to three times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
Subculture Ratio: approx. 1:3 to 1:8
The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping O-ring and cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Features and Benefits
These A549 cells are adherent, with a doubling time of approx. 22 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 18q21.1
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.