Documents

CLLS1127

Sigma-Aldrich

DLD1 Cells TP53 -/-

NACRES:
NA.81
Pricing and availability is not currently available.

biological source

human male colon (Source Disease: Colorectal adenocarcinoma)

Quality Level

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... TP53(7157)

General description

DLD1 Cells TP53-/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN double knock out modification.

This product corresponds to ATCC Cat. No. CCL-221.

Application

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1.3-1.10

The base medium for this cell line is RPMI, Catalog No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Catalog No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Catalog No. S8636, to a final concentration of 1 mM.

The cell freezing medium is DMSO 1X (Catalog Number C6164).

Features and Benefits

Zinc finger nuclease (ZFN) double knock out on chromosome 17p13.1

The DLD-1 cells are adherent, with a doubling time of approx. 20 hours.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, and PCR assay for cell line species confirmation.

Kit Components Also Available Separately

Product No.
Description
SDS

  • DLD1 Cells TP53 -/- 1 vial

  • DLD1 Control Cells 1 vial

Hazard Codes

B

WGK Germany

3

Certificate of Analysis
Certificate of Origin
Related Content
We have applied our revolutionary CompoZr Zinc Finger Nuclease technology to create an unparalleled range of genetically modified mammalian cell lines for use in areas, such as target validation, drug discovery and drug development.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.