Pricing and availability is not currently available.

Quality Level

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

OMIM accession no.

storage temp.


Gene Information

human ... ERBB2(2064), HER2(2064)

General description

MCF10A CELLS HER2 (-/-) EXON 2 are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN modification.

This cell line was derived from ATCC Catalog No. CRL-10317.


Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:8

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat. No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat. No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat. No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat. No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat. No. H6909, to a final concentration of 0.5 ug/mL.

Cell freezing medium - DMSO 1X, Cat. No. C6164.

Features and Benefits

These MCF10A cells are adherent with a doubling time of approx. 20 hours

Zinc finger nuclease (ZFN) modification on chromosome 17q21.1


Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Kit Components Also Available Separately

Product No.


  • MCF10A CELLS HER2 (-/-) EXON 2 1 vial


UN 3245 9

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
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