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CS0020

Sigma-Aldrich

phospho-p38 MAPK (pThr180/pTyr182) ELISA

sufficient for 96 determinations

Synonym(s):
p38 MAPK ELISA
Pricing and availability is not currently available.

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).

Application

p38 MAP kinase (MAPK), also known as a CDC-2-related protein kinase or CSBP (cytokine suppressive anti-inflammatory drug binding protein) regulates many cellular processes including inflammation, cell differentiation, cell growth and death. p38 MAP kinase is the human homolog of the S. cerevisiae gene Hog1, which is a MAPK required for growth under high-osmolarity conditions. The kit is designed to detect and quantify the levels of phosphorylated and non-phosphorylated p38 MAPK from cell lysates. It detects native and recombinant p38 protein.
phospho-p38 MAPK (pThr180/pTyr182) ELISA specifically measures human, monkey, and mouse p38 MAPK phosphorylated on threonine 180 and tyrosine 182.

Features and Benefits

The assays are a sensitive, quantitative, and economical alternative to traditional immunoblotting or bioassays. They are fast (4 hours), easy to perform (precoated plates, room temperature incubations, no proprietary equipment required) and the results are reproducible from lot-to-lot.

Analysis Note

The sensitivity is <0.8 units/mL, which is 10x more sensitive than immunoblotting.

Safety Statement

24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
C Weinbrenner et al.
Journal of molecular and cellular cardiology, 29(9), 2383-2391 (1997-09-23)
p38 mitogen-activated protein kinase (MAPK) is known to be activated after exposure to endotoxin, osmotic and environmental stress, and, most recently, during ischemia/reperfusion. We investigated whether ischemic preconditioning also causes phosphorylation of the activation sites on p38 MAPK. Three groups...

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