CS0030

Sigma-Aldrich

Senescence Cells Histochemical Staining Kit

sufficient for 100 tests

NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 100 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

detection method

colorimetric

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for b-galactosidase activity at pH 6. b-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells.

Application

For use in histochemical staining of b-galactosidase actifvity.

Biochem/physiol Actions

Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.

Legal Information

Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.

Kit Components Only

Product No.
Description

  • X-gal solution 4 mL

  • Staining Solution, 10X 15 mL

  • Fixation Buffer 10x 15 mL

  • Reagent B 1.5 mL

  • Reagent C 1.5 mL

  • Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL

Signal Word

Danger

Target Organs

Respiratory system

Supp Hazards

EUH032

Hazard Codes

C

Risk Statement

20/21/22-34-40-42/43

Safety Statement

23-26-36/37/39-45

RIDADR

UN 3316 9

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can Product CS0030, Senescence Cells Histochemical Staining Kit be used with fixed cells/tissues?

    Product CS0030, Senescence Cells Histochemical Staining Kit was designed for use with fresh (not-fixed) cells.  The kit contains a fixation buffer to fix the cells.  It is 10% formaldehyde, 1% glutaraldehyde in DPBS at 1X.  The cells will be fixed prior to staining.  The staining will show the beta-galactosidase activity that was present at the time of fixation.We tested the kit only on cells and we have no experience with tissue sections. The following papers use an assay for ßGAL on tissue sections which suggests that the kit will work with tissue sections.Castro P, et al., Interleukin-8 expression is increased in senescent prostatic epithelial cells and promotes the development of benign prostatic hyperplasia. Prostate, 60(2), 153-159 (2004).Chen, Z., et al., Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Nature, 436, 725-730 (2005).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Natalja E Fedorovich et al.
Biomaterials, 30(3), 344-353 (2008-10-22)
Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules....
G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is...
Michael J MacLeod et al.
Journal of fish diseases, 41(9), 1359-1372 (2018-06-09)
In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and...
Phuc H Pham et al.
In vitro cellular & developmental biology. Animal, 53(1), 20-32 (2016-09-03)
As diseases and abnormalities of the heart can interfere with the aquaculture of Atlantic salmon, the heart was investigated as a source of cell lines that could be used to study the cellular basis of these conditions. An Atlantic salmon...
B van der Loo et al.
Experimental cell research, 241(2), 309-315 (1998-06-25)
A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures...
Articles
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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