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CS0040

Sigma-Aldrich

phospho-p53 (pSer15) ELISA human

sufficient for 96 multiwell tests

Synonym(s):
p53 ELISA
Pricing and availability is not currently available.

usage

sufficient for 96 multiwell tests

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... TP53(7157)

General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).

Application

The ELISA is specific for the measurement of human p53 protein phosphorylated at serine 15 in cell lysates. The assay does not detect phosphorylated p53 in mouse or rat lysates. Phosphorylation of p53 at serine 15 by ATM, ATR, and DNAPK leads to a reduced interaction with its negative regulator, MDM2, and accumulation of p53 protein.
Designed for the in vitro quantitative determination of human p53 protein in cell lysates. The assay does not react with p53 in mouse and rat.

Biochem/physiol Actions

p53 gene is highly conserved and expressed in normal tissues. It is the most commonly mutated gene in human cancer and more then 500 gene mutations have been described in various types of malignancies, hematologic as well as solid tumors. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells. Thus, p53 plays a vital role in suppressing the development of cancer.

Features and Benefits

p53 ELISAs offer a fast (total assay time 4 hours), sensitive (<50 pg/mL for total p53 and <0.9 units/mL for phosphospecific p53, which is 8x more sensitive than immunoblotting), and an easy alternative to immunoblotting or other bioassays.

Analysis Note

The sensitivity is <0.9 units/mL for phosphospecific p53, which is 8x more sensitive than immunoblotting. The specificity was confirmed by peptide competition in which only the peptide containing phosphorylated serine 15 blocks the ELISA signal.

Hazard Codes

B

Safety Statement

24/25

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
p53, the cellular gatekeeper for growth and division.
A J Levine
Cell, 88(3), 323-331 (1997-02-07)

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