A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).
The ELISA is specific for the measurement of human p53 protein phosphorylated at serine 15 in cell lysates. The assay does not detect phosphorylated p53 in mouse or rat lysates. Phosphorylation of p53 at serine 15 by ATM, ATR, and DNAPK leads to a reduced interaction with its negative regulator, MDM2, and accumulation of p53 protein.
Designed for the in vitro quantitative determination of human p53 protein in cell lysates. The assay does not react with p53 in mouse and rat.
p53 gene is highly conserved and expressed in normal tissues. It is the most commonly mutated gene in human cancer and more then 500 gene mutations have been described in various types of malignancies, hematologic as well as solid tumors. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells. Thus, p53 plays a vital role in suppressing the development of cancer.
Features and Benefits
p53 ELISAs offer a fast (total assay time 4 hours), sensitive (<50 pg/mL for total p53 and <0.9 units/mL for phosphospecific p53, which is 8x more sensitive than immunoblotting), and an easy alternative to immunoblotting or other bioassays.
The sensitivity is <0.9 units/mL for phosphospecific p53, which is 8x more sensitive than immunoblotting. The specificity was confirmed by peptide competition in which only the peptide containing phosphorylated serine 15 blocks the ELISA signal.