CS0170

Sigma-Aldrich

Thioredoxin Reductase Assay Kit

 kit sufficient for 100 assays (1 mL)

NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

usage

 kit sufficient for 100 assays (1 mL)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)

General description

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Application

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Features and Benefits

  • An easy and simple method
  • The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
  • The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
  • The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

Kit Components Only

Product No.
Description

  • Assay Buffer 5x for Thioredoxin Reductase 30 mL

  • Thioredoxin Reductase 10 μg

  • Thioredoxin Reductase Inhibitor Solution .05 mL

  • 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg

  • NADPH 25 mg

  • Dimethyl Sulfoxide (DMSO) 7.5 mL

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificate of Analysis
Certificate of Origin
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