Documents

CS0210

Sigma-Aldrich

Prostaglandin EIA Kit

Prostaglandin E1 (PGE1), sufficient for 96 assays

Pricing and availability is not currently available.

usage

sufficient for 96 assays

shipped in

dry ice

storage temp.

−20°C

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General description

Competitive enzyme immunoassays (EIA) are employed for the quantitative determination of prostaglandin E1 (PGE1) and prostaglandin E2 (PGE2) in biological fluids. In the assays, the specific protein in the sample competes with a fixed amount of alkaline phosphatase-labeled conjugate for binding sites on the antibody. The intensity of the bound yellow color read at 405 nm is inversely proportional to the concentration of prostanoid in the standards or samples. The measured optical density is used to calculate the concentration.

Biochem/physiol Actions

Prostaglandins and thromboxane are products of the cyclooxygenase pathway and are synthesized in response to immediate need and are not stored in significant amounts for later release. Prostanoids mediate various biological functions in humans, including platelet aggregation, gastric mucosa protection, renal homeostasis, vascular homeostasis, uterine function, embryo implantation and labor and sleep/wake cycle.
PGE1 causes vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has insulin-like actions. Major prostaglandin in semen.

Features and Benefits

The assays specifically measure each target lipid with negligible cross-reactivity with 20-25 related eicosanoid compounds tested (from <0.1 to 2%). A standard curve is run in each assay, precoated plates eliminate the coating step, all incubations proceed at room temperature, any commercially available microwell plate reader may be used and reagents are color coded for easy use. The results are available in 4 hours. Performance characteristics show low intra- and inter-assay variability, linear standard curve and recovery of samples ranging from 99-111%.

Principle

The sensitivity of PGE1 EIA is less than 6.06 pg/mL, sample recovery in different matrices ranges from 87% to 106% and the control parameters were established.

Hazard Codes

T

Risk Statement

5-23/24/25-32-34-52/53

Safety Statement

26-36/37/39-45

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
T Koga et al.
Acta anaesthesiologica Scandinavica, 46(8), 987-993 (2002-08-23)
The inhibitory effect of prostaglandin E1 (PGE1) on platelet aggregation is considered an important characteristic of this agent. However, the concentration of PGE1 to inhibit aggregation in vitro is higher than those of clinical use (1 ng/ml). To clarify whether...

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