The p38 MAPK Assay Kit provides an easy method to assay p38 MAPK activity and explore new p38MAPK stimuli, inhibitors, and activators. The kit provides all the reagents required for the straightforward detection and measurement of p38 MAPK activity in cell lysates, tissue homogenates, column fractions, or the purified enzyme. In addition, the kit provides a specific p38 MAPK inhibitor to enable the verification of the specificity of the kinase activity observed.
The assay is based on immunoprecipitation of the active form of p38α kinase and detection of its phosphorylation activity on the substrate ATF2 by immunoblotting, without the need for a secondary antibody. An alternative protocol for radioactive measurement of p38 MAPK activity is also provided.
The mitogen-activated protein kinase (MAPK) p38 pathway is a signaling cascade activated by proinflammatory stimuli and cellular stresses, playing a critical role in the translational regulation of proinflammatory cytokine synthesis.