CS0260

Sigma-Aldrich

Glutathione Assay Kit

sufficient for 700 assays

NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 700 assays

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... GSR(2936)

General description

The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm.

Application

Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay. It also has been used to determine different oxidative stress parameters in rats.

Biochem/physiol Actions

Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues
Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases.

Features and Benefits

  • A quick and simple method
  • The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)
  • The kit includes a deproteination reagent for biological samples

Kit Components Only

Product No.
Description

  • Assay Buffer 5x 30 mL

  • DTNB 8 mg

  • DMSO 7.5 mL

  • Glutathine Reductase 20 U

  • L-Glutathione Reduced 300 μg

  • NADPH 25 mg

  • 5-Sulfosalicylic Acid 2.5 g

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Codes

Xn

Risk Statement

22-36/37/38

Safety Statement

26

WGK Germany

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I calculate DA412/min when using Product CS0260, Glutathione Assay Kit?

    The delta A412/min (sample) stands for the average increase in value per min of the OD over a 4 minute period. The delta A412/min (1 nmole) can be calculated by regression analysis of the points of the standard curve. The standard curve should be obtained by plotting a graph of delta A412/min versus nmole GSH.

  4. Is Product CS0260, Glutathione Assay Kit, for determination of reduced glutathione, oxidized glutathione, or both?

    This kit is designed to measure total glutathione. The glutathione reductase included in the kit will reduce oxidized glutathione present in the sample.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Melissa Herwig et al.
Frontiers in physiology, 11, 240-240 (2020-05-01)
The giant protein titin performs structure-preserving functions in the sarcomere and is important for the passive stiffness (Fpassive) of cardiomyocytes. Protein kinase D (PKD) enzymes play crucial roles in regulating myocardial contraction, hypertrophy, and remodeling. PKD phosphorylates myofilament proteins, but...
Role of thiol-complex formation in 2-hydroxyethyl- methacrylate-induced toxicity in vitro
Samuelsen,J et al.
Journal of Biomedical Materials Research, 96A, 385-401 (2011)
Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells
Sweeney EE, et al.
International Journal of Oncology, 44(5), 1529-1538 (2014)
Metallothionein-1G facilitates sorafenib resistance through inhibition of ferroptosis
Sun X, et al.
Hepatology, 64(2), 488-500 (2016)
Glutathione: Physiological and clinical relevance
Pizzorno JE and Katzinger JJ
Journal of restorative medicine, 1(1), 24-37 (2012)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.