Documents

CS0270

Sigma-Aldrich

Hydrogen Peroxide Colorimetric Assay

sufficient for 96 multiwell tests

Synonym(s):
H2O2 Colorimetric Assay
Pricing and availability is not currently available.

usage

sufficient for 96 multiwell tests

shipped in

dry ice

storage temp.

−20°C

Biochem/physiol Actions

Hydrogen peroxide is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. Functioning through NFκB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, arteriosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down′s Syndrome.

Features and Benefits

This very simple colorimetric H2O2 assay does not require sample extraction or lysis, involves one-step procedure with one reagent, and the results are available within 30 minutes. The assay is sensitive and detects H2O2 at 51.25 ng/mL when diluted in buffer. Performance characteristics show intra-assay variability from 3-10% and inter-assay variability from 1.7-5.7%. Dilutions of H2O2 are run multiple times in the same assay and the sample standard curve had a slope of 0.9342 with a coefficient of variation of 0.9983. Plasma, urine, and serum samples spiked with H2O2 and diluted 1:64 gave >90% recovery.

Caution

The standard is light sensitive. Protect from light. Hydrogen peroxide color reagent must be frozen at −20 °C and during use must be kept at 4 °C. Do not use reagents past kit shelf life.

Hazard Codes

Xn

Risk Statement

22-36/38-41

Safety Statement

26-36-39

RIDADR

UN 3264 9/PG 3

Certificate of Analysis
Certificate of Origin
Ahmed M El-Lekawy et al.
Journal of pediatric gastroenterology and nutrition, 69(6), 710-718 (2019-11-26)
Alanylglutamine (AG) is a dipeptide that fuels enterocytes and has a coadjuvant role during gut healing. The current study aimed to investigate the potential ulcer-healing effect of AG in indomethacin-induced gastropathy. Animals (n = 10 rats/group) were randomly allocated into 5 groups. Gastric...

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