Hydrogen Peroxide Colorimetric Assay

sufficient for 96 multiwell tests

H2O2 Colorimetric Assay
Pricing and availability is not currently available.


sufficient for 96 multiwell tests

shipped in

dry ice

storage temp.


Biochem/physiol Actions

Hydrogen peroxide is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. Functioning through NFκB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, arteriosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down′s Syndrome.

Features and Benefits

This very simple colorimetric H2O2 assay does not require sample extraction or lysis, involves one-step procedure with one reagent, and the results are available within 30 minutes. The assay is sensitive and detects H2O2 at 51.25 ng/mL when diluted in buffer. Performance characteristics show intra-assay variability from 3-10% and inter-assay variability from 1.7-5.7%. Dilutions of H2O2 are run multiple times in the same assay and the sample standard curve had a slope of 0.9342 with a coefficient of variation of 0.9983. Plasma, urine, and serum samples spiked with H2O2 and diluted 1:64 gave >90% recovery.


The standard is light sensitive. Protect from light. Hydrogen peroxide color reagent must be frozen at −20 °C and during use must be kept at 4 °C. Do not use reagents past kit shelf life.

Hazard Codes


Risk Statement


Safety Statement



UN 3264 9/PG 3

Certificate of Analysis
Certificate of Origin
Ahmed M El-Lekawy et al.
Journal of pediatric gastroenterology and nutrition, 69(6), 710-718 (2019-11-26)
Alanylglutamine (AG) is a dipeptide that fuels enterocytes and has a coadjuvant role during gut healing. The current study aimed to investigate the potential ulcer-healing effect of AG in indomethacin-induced gastropathy. Animals (n = 10 rats/group) were randomly allocated into 5 groups. Gastric...

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