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CS0300

Sigma-Aldrich

Caspase 9 Fluorescein (FLICA) Assay

sufficient for 100 tests

Synonym(s):
Caspase Fluorescein (FLICA) Assay
Pricing and availability is not currently available.

usage

sufficient for 100 tests

storage temp.

2-8°C

Application

Fluorometric detection of active cellular caspase 9 using FAM-LEHD-FMK cell-permeable, non-toxic fluorochrome inhibitor of caspase 9 that binds covalently to the active caspase 9 and is visualized by fluorometry, fluorescence microscopy, or flow cytometry.
FLICA Caspase Assay detects active caspases inside the cell using novel Fluorochrome Inhibitor of Caspases (FLICA)-cell permeable and non-cytotoxic caspase inhibitors. Once inside the cell, the FLICA probe covalently binds to a reactive cysteine residue on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. Bound FLICA is retained within the cell, while any unbound reagent is washed away. The bound FLICA emits a green fluorescent signal, which is a direct measurement of the number of active caspase molecules that are present in the cell at the time the reagent was added.

Features and Benefits

The FLICA Assays offer:
  • Potent, cell-permeable and non-toxic fluorochrome inhibitor
  • Preferential detection of selected caspases based on inhibitor specificity
  • A direct measure of apoptosis expressed as the number of active caspase enzymes present in the cell

Caution

Once reconstituted, the unused 150X FLICA stock should be stored in aliquots at −20 °C protected from light.

Kit Components Only

Product No.
Description

  • FAM-LEHD-FMK FLICA Reagent 4 vial(s)

  • Wash Buffer, Concentrate 10X 60 mL

  • Fixative 6 mL

  • Propidium iodide 1 mL

  • Hoechst Stain 33342 1 mL

Hazard Codes

Xn

Risk Statement

20/21/22-40-43

Safety Statement

36/37

Certificate of Analysis
Certificate of Origin
Vanessa S Marsden et al.
Nature, 419(6907), 634-637 (2002-10-11)
Apoptosis is an evolutionarily conserved cell suicide process executed by cysteine proteases (caspases) and regulated by the opposing factions of the Bcl-2 protein family. Mammalian caspase-9 and its activator Apaf-1 were thought to be essential, because mice lacking either of...

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