Documents

CS0380

Sigma-Aldrich

JNK 1&2 Activity Assay Kit

sufficient for 50 reactions

Synonym(s):
c-Jun N-terminal kinase activity assay kit
Pricing and availability is not currently available.

usage

sufficient for 50 reactions

shipped in

dry ice

storage temp.

−20°C

Application

JNK1&2 Activity Assay Kit offers an easy method to assay JNK1&2 activity and to explore new JNK stimuli, inhibitors and activators. The kit provides all the reagents required for straightforward detection and measurement of JNK activity in cell lysates, tissue homogenates, column fractions or purified kinases.The assay detects phosphorylated JNK 1&2 based on immunoprecipitation of the kinase using anti-JNK antibodies, and a detection of the phosphorylation activity of its substrate, ATF2, by immunoblotting. Alternative radioactive protocol is included.

Kit Components Only

Product No.
Description

  • Anti-c-Jun N-terminal Kinase .2 mL

  • Monoclonal Anti-phospho ATF2 (pThr69,71)−Peroxidase 1 mL/vial

  • Assay Buffer For Kinase Activity 1 mL

  • Wash Buffer 10X 8 mL

  • EZviewTM Red Protein A Affinity Gel, 1 mL

  • ATF2 .5 mg

Safety Statement

23-24/25

Certificate of Analysis
Certificate of Origin
D R Phelan et al.
The Journal of biological chemistry, 276(14), 10801-10810 (2001-03-30)
MAP kinase signaling pathways are important mediators of cellular responses to a wide variety of stimuli. Signals pass along these pathways via kinase cascades in which three protein kinases are sequentially phosphorylated and activated, initiating a range of cellular programs...

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